Journal:

Article Title: Defining Parameters for Homology-Tolerant Database Searching

doi:

Figure Lengend Snippet: Results from 25 samples comparing peptide sequences obtained using PEAKS with the actual known peptide sequence from the database. The bars on the left show the percent occurrence of 1, 2, 3, or 4 mismatched amino acid in the peptides. The bars on the right indicate the influence of the relative location of amino acid substitutions on the incidence of mismatch.

Article Snippet: The presence of the sequence data should increase the speed and scope of a database search, but the overall throughput is severely constrained by the interpretation step.3,5 However, compensation for the time required to de novo sequence a peptide may be gained by an increase in identification of proteins from divergent species by combining peptide mass with partial sequence information12 or using automated algorithms to derive sequence information.10,13,14 In this series of experiments, sequence information was obtained by employing the automated de novo sequencing program PEAKS (Studio 2.0, Bioinformatics Solutions, Ontario, Canada).

Techniques: Sequencing